Identification of a Methylation Pattern in the SNRPN Gene Promoter and its Association with Semen Abnormality Among Iraqi Males

Infertility considered as a multifactorial condition; the small nuclear ribonucleoprotein polypeptide N (SNRPN) gene is an imprinted gene. However, abnormal imprinting of this due to methylation may result in function or silencing Main aim study investigate present at promoter and its role risk factor for male infertility. Sixty- three infertile males with age mean (32.28 ± 6.88 years) 13 fertile control (34.07 6.52 were investigated. Whole genomic DNA was extracted, integrity checked using β-globin internal control. The targeted region amplified by polymerase chain reaction (PCR) technique. In addition, SNRPN gene's qualitatively detected Real time (qPCR) utilizing two sets primers: methylated un-methylated. Results reveled that all 63 experiencing decrease sperm concentration 9.42 8.70 million/ml, reduced progressive motility 2.89 5.45% well strange morphology 27.06 16.50%, while values group are normal. results current investigation showed hypermethylated some samples 22.7%, somewhat others 20.4%, unmethylated other 56.8% from samples, none had any methylation. These findings suggest be associated negative changes semen parameters, which could lead